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Lipidomic analysis of isolated monocytes and neutrophils by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS) yielded a primary data set of 521 monocyte and 706 neutrophil lipid species, which were normalized to an extensive set of internal standards as previously reported (13, 14) (see Methods for a full description)

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Through a gene duplication event, this gene—which has been shown to exhibit some ice-binding activity of its own—evolved into an effective AFP gene

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